Introduction to ACSF2
ACSF2 (Acyl-CoA Synthetase Family Member 2) is a mitochondrial matrix enzyme that plays an essential role in medium- to long-chain fatty acid activation through the synthesis of acyl-CoA esters. This activation is a key step in initiating β-oxidation, which contributes to ATP generation via mitochondrial respiration. According to the NCBI Gene Database, ACSF2 is expressed in energy-demanding tissues like the liver, heart, and skeletal muscle.
The role of ACSF2 has also been investigated in projects such as the Human Protein Atlas proteinatlas.org, which reveals its localization in the mitochondria and correlation with genes involved in oxidative phosphorylation.
What Is an ACSF2 Polyclonal Antibody?
A polyclonal antibody against ACSF2 is developed by immunizing an animal (typically rabbit or goat) with peptides derived from conserved domains of the ACSF2 protein. These antibodies are polyclonal, meaning they recognize multiple epitopes on the antigen, enhancing detection capability across native, denatured, or fragmented forms of the protein.
The antibody is purified and validated for use in:
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Western blot (WB)
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Immunohistochemistry (IHC)
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Immunocytochemistry (ICC)
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ELISA
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Mitochondrial protein quantification assays
Detailed guidance for polyclonal antibody usage is offered through portals like the NIH Office of Research Infrastructure Programs and Antibody Registry.
ACSF2 in Mitochondrial Lipid Metabolism
ACSF2 catalyzes the formation of acyl-CoA from ATP, CoA, and fatty acids, thus priming them for entry into the β-oxidation pathway. This reaction is crucial for the maintenance of lipid homeostasis and energy flexibility in metabolically active cells. Mitochondrial acyl-CoA levels, regulated by ACSF2, also influence signaling pathways such as AMPK, PPAR, and CPT1.
For insights on mitochondrial fatty acid oxidation, the National Center for Biotechnology Information (NCBI) provides in-depth reviews.
Research Applications of ACSF2 Polyclonal Antibody
. Mitochondrial Metabolic Studies
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Used to validate mitochondrial protein purity in fractionation protocols.
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Detects changes in fatty acid activation enzymes under nutrient-stress conditions.
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Correlates with data from MitoCarta 3.0, a mitochondrial protein inventory resource.
. Comparative Tissue Profiling
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Enables researchers to compare ACSF2 expression across tissues using Western blot or immunohistochemistry.
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Differential expression helps in understanding tissue-specific lipid oxidation patterns. See reference tissues in The Human Protein Atlas.
. Knockout and Overexpression Validation
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Serves as a marker to confirm gene editing results in CRISPR-modified cell lines or animal models.
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Supports phenotypic studies in metabolic pathway modulation. Refer to Mouse Genome Informatics for transgenic models.
. Cell Line Studies
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ACSF2 antibody is commonly used in HepG2, C2C12, and H9c2 cell models to investigate mitochondrial energy metabolism.
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Useful in experiments involving nutrient starvation, fatty acid supplementation, or mitochondrial uncoupling.
Protocol Compatibility
ACSF2 Polyclonal Antibody is optimized for use in:
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SDS-PAGE and WB: Detects ~73 kDa band under reducing conditions.
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IHC and ICC: Localizes to mitochondria in fixed tissues or cells.
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Peptide Blocking: Confirm specificity with immunogen-based peptide inhibition.
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Cross-reactivity: Validated across human, mouse, and rat models.
For technical optimization, consult NIH Image Resources and NCBI Protocol Exchange.
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Resources for Experimental Design
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GenBank – mRNA sequence and translation products.
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ClinicalTrials.gov – Related human studies on mitochondrial metabolism.
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PubMed – Articles involving ACSF2 expression and function.
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NIH Toolbox – Reagents, validation protocols, and method references.
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NSF Award Search – Grants related to lipid and mitochondrial enzyme research.
Product Availability and Technical Support
The ACSF2 Polyclonal Antibody is distributed by Gentaur, available in 100 µg, 200 µg, or 100 µL volumes. The product includes:
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Technical data sheet
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SDS (Safety Data Sheet)
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Certificate of Analysis (COA)
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Immunogen sequence
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Host species and isotype
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Recommended dilution for WB/IHC/ICC
Contact Gentaur via www.gentaur.com for bulk orders, custom conjugation, and validation reports. Distributors can also provide shipping conditions, lead times, and storage temperature guidance.
Conclusion: Why ACSF2 Polyclonal Antibody Matters
ACSF2 Polyclonal Antibody is an essential reagent for studies involving lipid metabolism, energy regulation, and mitochondrial enzymology. Its wide compatibility with major detection platforms and reproducible performance across multiple models make it ideal for both exploratory and routine investigations.
Researchers studying mitochondrial bioenergetics, fatty acid dynamics, and tissue-specific metabolic programming will find this antibody a dependable tool for visualizing and quantifying ACSF2 in complex biological samples.
Use of well-validated antibodies like ACSF2 enhances the transparency, reproducibility, and impact of molecular biology experiments—core goals endorsed by agencies like the National Institutes of Health (NIH) and the U.S. Department of Health and Human Services.
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acsf2 antibody western blot
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polyclonal antibody for fatty acid metabolism
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acsf2 immunohistochemistry validation
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antibody for mitochondrial lipid enzymes
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acyl-coa synthetase detection in liver tissue
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western blot detection of fatty acid metabolism proteins
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energy metabolism antibodies in cell lines
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high-yield mitochondrial antibody detection
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lipid oxidation research antibody
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acsf2 protein mitochondrial localization